Capillary electrophoresis: Applicability and method validation for biorefinery analytics
Kaijanen, Laura (2015-09-18)
Väitöskirja
Kaijanen, Laura
18.09.2015
Lappeenranta University of Technology
Acta Universitatis Lappeenrantaensis
Julkaisun pysyvä osoite on
https://urn.fi/URN:ISBN:978-952-265-846-3
https://urn.fi/URN:ISBN:978-952-265-846-3
Tiivistelmä
Wood-based bioprocesses present one of the fields of interest with the most potential in
the circular economy. Expanding the use of wood raw material in sustainable industrial
processes is acknowledged on both a global and a regional scale.
This thesis concerns the application of a capillary zone electrophoresis (CZE) method
with the aim of monitoring wood-based bioprocesses. The range of detectable
carbohydrate compounds is expanded to furfural and polydatin in aquatic matrices. The
experimental portion has been conducted on a laboratory scale with samples imitating
process samples.
This thesis presents a novel strategy for the uncertainty evaluation via in-house validation.
The focus of the work is on the uncertainty factors of the CZE method. The CZE
equipment is sensitive to ambient conditions. Therefore, a proper validation is essential
for robust application.
This thesis introduces a tool for process monitoring of modern bioprocesses. As a result,
it is concluded that the applied CZE method provides additional results to the analysed
samples and that the profiling approach is suitable for detecting changes in process
samples.
The CZE method shows significant potential in process monitoring because of the
capability of simultaneously detecting carbohydrate-related compound clusters. The
clusters can be used as summary terms, indicating process variation and drift.
the circular economy. Expanding the use of wood raw material in sustainable industrial
processes is acknowledged on both a global and a regional scale.
This thesis concerns the application of a capillary zone electrophoresis (CZE) method
with the aim of monitoring wood-based bioprocesses. The range of detectable
carbohydrate compounds is expanded to furfural and polydatin in aquatic matrices. The
experimental portion has been conducted on a laboratory scale with samples imitating
process samples.
This thesis presents a novel strategy for the uncertainty evaluation via in-house validation.
The focus of the work is on the uncertainty factors of the CZE method. The CZE
equipment is sensitive to ambient conditions. Therefore, a proper validation is essential
for robust application.
This thesis introduces a tool for process monitoring of modern bioprocesses. As a result,
it is concluded that the applied CZE method provides additional results to the analysed
samples and that the profiling approach is suitable for detecting changes in process
samples.
The CZE method shows significant potential in process monitoring because of the
capability of simultaneously detecting carbohydrate-related compound clusters. The
clusters can be used as summary terms, indicating process variation and drift.
Kokoelmat
- Väitöskirjat [1099]