Bisphenol A (BPA) removal from aqueous solution by laccase from the white-rot fungi Trichoderma atroviride & Fomes fomentarius
Zia, Muhammad Umer (2026)
Diplomityö
2026
School of Engineering Science, Kemiantekniikka
Kaikki oikeudet pidätetään.
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi-fe20260622100782
https://urn.fi/URN:NBN:fi-fe20260622100782
Tiivistelmä
Bisphenol A (BPA) is an endocrine-disrupting compound that can enter aquatic environments through wastewater, landfill leachate, industrial discharge and diffuse urban sources. Because BPA contains phenolic groups, fungal laccases offer a potential biological treatment route for its transformation. This thesis investigated the production of extracellular laccase by Trichoderma atroviride and Fomes fomentarius and evaluated the use of crude F. fomentarius laccase for BPA removal from aqueous solution.
In T. atroviride, despite positive laccase screening, significant laccase production was not achieved under different liquid and semi solid conditions. In comparison, F. fomentarius produced 7442 U/L laccase (specific activity 9.3 U/mg) on day 13 under semi-solid fermentation using wheat bran support-substrate and defined glucose based medium. SDS-PAGE showed a main protein band between 55 and 70 kDa, while Native-PAGE activity staining confirmed the presence of active laccase. The highest observed BPA removal was 88.8% after 30 min at pH 5, 220.7 U/L laccase activity, 30 °C, 125 rpm and 50 mg/L initial BPA concentration. This showed that crude F. fomentarius laccase could remove BPA rapidly without adding an external redox mediator. On the other hand, phytotoxicity assessment using L. sativa showed that BPA removal did not result in detoxification under the tested conditions.
In T. atroviride, despite positive laccase screening, significant laccase production was not achieved under different liquid and semi solid conditions. In comparison, F. fomentarius produced 7442 U/L laccase (specific activity 9.3 U/mg) on day 13 under semi-solid fermentation using wheat bran support-substrate and defined glucose based medium. SDS-PAGE showed a main protein band between 55 and 70 kDa, while Native-PAGE activity staining confirmed the presence of active laccase. The highest observed BPA removal was 88.8% after 30 min at pH 5, 220.7 U/L laccase activity, 30 °C, 125 rpm and 50 mg/L initial BPA concentration. This showed that crude F. fomentarius laccase could remove BPA rapidly without adding an external redox mediator. On the other hand, phytotoxicity assessment using L. sativa showed that BPA removal did not result in detoxification under the tested conditions.